Hi Joe,
1. In the average office environment I typically see around 96% reception for a 4 Hz channel, though this can vary drastically depending on the RF environment.
2. This can be set in the channel assignment, and is a different hex value than assigning a bi-directional master channel. I strongly recommend against doing this even if your slave channels never send messages to the master channel as this disables ANT master coexistence.
3. This depends on the device you are using but typically if you were using an older AP2 or C7 module the serial bus would restrict you to ~200 Hz. Actual OTA coexistence typically can sustain up to ~300 Hz of total master channel transmissions before timeslots begin constantly overlapping.
If you are the only master transmitting, and you are not doing bi-directional communication, and you are running an SoC only (N550), then you could easily exceed 500 Hz, but this would require quite a bit of testing.